Rambod Abiri1, Noor Azmi Shaharuddin1*, Mahmood Maziah1, Zetty Norhana Balia Yusof1, Narges Atabaki2, Mahbod Sahebi3, and Parisa Azizi3
Seed priming is a useful technique which improves seed germination parameters. The present investigation was conducted to evaluate some of the most important germination parameters such as total germination, germination rate, mean germination time, average speed, germination percentage (GP), germination vigor index (GVI), relative frequency, plumule fresh and dry weight, radical fresh and dry weight, plumule and radical length, leaf and root proline content of six Indica rice (Oryza sativa L.) varieties, including MR219, MR219-4, MR219-9, MR220, MR 159, and MR 211 under hydro-, hormonal- and polyethylen glycol (PEG)- priming conditions. The highest germination parameters were achieved under the hydro-priming treatment, at 18 h (100%), 6 h (100%), 18 h (90.3%), 12 h (91.6%), 18 h (86.6%), and 18 h (78.3%) for the genotypes MR219, MR219-4, MR219-9, MR220, MR 159, and MR 211, respectively. The best germination feedback of the rice varieties under the hormonal priming were observed in 50 mg L-1 abscisic acid (ABA), 10 mg L-1 gibberellic acid-3 (GA3), 50 mg L-1 indole-3-acetic acid (IAA), 50 mg L-1 GA3, 100 mg L-1 ABA, and 10 mg L-1 GA3 for the genotypes MR219, MR219-4, MR219-9, MR220, MR 159 and MR 211, respectively. The rice varieties showed different responses to various levels of PEG in which MR219, MR219-4 and MR219-9 responded positively to higher PEG levels, while MR220, MR159 and MR 211 showed better feedback under lower PEG concentrations. These outcomes comply with the higher tolerance of MR219, MR219-9 and MR219-4 varieties to drought stress.
Key words: PEG-priming, hormonal priming, hydropriming, Oryza sativa, proline.
1Universiti Putra Malaysia, Faculty of Biotechnology and Biomolecular Sciences, 43400 UPM Serdang, Selangor DE, Malaysia. *Corresponding author (email@example.com).
2Islamic Azad University, Science and Research Branch, 1477893855, Tehran, Iran.
3Universiti Putra Malaysia, Institute of Tropical Agriculture, 43400 UPM Serdang, Selangor DE, Malaysia.