Comparison between a tolerant and non-tolerant wheat cultivar in their Barley YeIlow Dwarf Virus content, by ELISA

Guido Herrera M.1 y Marizol Muñoz R.1

The Enzyme-Linked Inmunosorbent Assay (ELISA) was used to study the BYDV content in leaves, stems and roots of the tolerant (Tolbay-INIA) and the non-tolerant (Mexifén) wheat cultivars in twogrowth stages, E.7and E.10.5
Plan-ts were inoculated using Ropalosiphum padi and Sitobion avenae aphids, contaminated with a “PAV-like” Chilean isolation, when they reached the E.5 growth stage.
The results indicated:
a. BYDV content in leaves of the tolerante cv. increased from the E.7 to the E.10.5 growth stages. ln the non-tolerant cv., this content decreased during the same period.
b. BYDV content in the stems and roots of the tolerant cv. did not vary; while in the non-tolerant cv. this content increased, from the E.7 to the E.1 0.5 stages.
c. At the E.10.5 stage, the non-tolerant cv. showed a lower content in leaves and a higher content in roots than the tolerant one; BYDV conteni in the stems was higher for the non-tolerant, but this difference wasnot statistical.
d. Yield decreased 59% in the non-tolerant cv., and only 11% in the tolerant. Losses were also higher for the non-tolerant cv. in grain weight, number of grains/spike, and harvest index.

1 Estación Experimental La Platina (INIA), Casilla 5427, Santiago, Chile.