Identification of internal fungi, present in bean seeds

Jorge Nitsche M.1 y Claudio Cafati K.1

Seed samples of bean cultivars currently grown in Chile were harvested from fields in commercial farms and experimental stations (INIA), located between the Metropolitan Region and the VIII Region, at different phenological periods of the crop: 15, 30 and 45 days after physiological maturity.
The seed infernal fungi were subjected fo in vitro and paper towel analyses and their counts were recorded. The isolated fungi were used in pathogenicity tests.
Results showed excellent sanitary conditions of the seeds, specially in samples ofthesecond harvest period.
The isolated fungi, that demonstrated to be pathogens on beans, were: Rhizopus stolonifer Lind, Rhizoctonia soiani Kuhn, Botrytis cinerea Pers.; and Fusarium oxysporum Schlecht. Some saprophytic fu ngi genera were also isolated; Alternaria, Stemphylium, Aspergillus, Penicillium, Epicoccum, Sclerotium, Macrophomina, HeIminthosporium and Chaetomium.

1 Estación Experimental La Platina (INIA), Casilla 5427, Santiago, Chile.