ChileanJAR

A biological method for gibberellins determination (A₃) was tested. This compound produces the extension of the cucumber hypocotyl segments (Cucumis sativus var. Inglés) if their cotyledons are not excised.
This bioassay is specific for gibberellic acid (A₃). Kinetins (furfuryl amino purine) and indolacetic acid practically do not induce growth.
The sensibility of the method was studied and it is coácluded that is sensitive to a concentration of l X 10^-9 g/mI G. A.
Investigations were carried out on the effects of temperature, incubation time and seedling length to obtain the maximun growth.