ABSTRACT.


Jorge Fuentes Z.1 y Claudio Ciudad B.2
 

A method for the extraction of proteins from maize opaque-2 and non opaque on the basis of differential solubility was studied.
The fractions extracted were subjected to electrophoresis.
Maize opaque-2 showed an increase in glutelin, and a decrease in zein with respect to maize non-opaque, in the extraction process.
Cellulose polyacetate as a support of electrophoresis is rapid, easy to handle and of a high resolution.
Good separation of the water-soluble proteins (albumins) and salt-soluble proteins (globulins) was obtained. both proteins show a similar electropherogram in maize opaque-2 and non-opaque.

Keywords:
1Químico Farmacéutico, becado por CONICYT, Laboratorio Central de Bromatologia, Estación Experimental La Platina, Instituto de Investigaciones Agropecuarias, Casilla 5427, Santiago, Chile.
2Bioquímico, Laboratorio Central de Bromatología, Estación Experimental La Platina, Instituto de Investigaciones Agropecuarias, Casilla 5427, Santiago, Chile.