In vitro propagation of cedar (Cedrela odorata L.) from juvenile shoots

Rolando García-Gonzáles1*, Miladys Delgado2, Yailín González2, Aníbal González2, Miguel Garriga3, Peter D.S. Caligari3, Basilio Carrasco4, and Karla Quiroz1

Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium supplemented with 2 mg L-1 6-bencilaminopurine and 3 mg L-1 naphthaleneacetic acid. This medium resulted in 100% shoot development from the in vitro node explants with a 3.93 cm mean height. Rooting was also stimulated 6 wk after individualization of the regenerated plants on the same micropropagation medium with a mean of 3.9 roots per plant. In vitro plants did not show morphologic differences when compared to ex vitro seeds.

Keywords: Organogenesis, in vitro propagation, Cedrela odorata, in vitro rooting, plant growth regulators.
1Universidad Católica del Maule, Facultad de Ciencias Agrarias y Forestales, Campus San Miguel. Av. San Miguel 3605, Casilla 617, Talca. *Corresponding author (rgarciag@ucm.cl).
2Estación Experimental Forestal de Camagüey, P.O. Box 405, C.P. 70100, Camagüey, Cuba.
3Universidad de Talca, Instituto de Biología Vegetal y Biotecnología, 2 Norte 845, Talca, Chile.
4Pontificia Universidad Católica de Chile, Facultad de Agronomía e Ingeniería Forestal, Vicuña Mackenna 4860, Casilla 306, Correo 22, Santiago, Chile.