ABSTRACT
Double haploid plants generated by intergeneric wheat x maize crosses

Claudio Jobet1, Javier Zuñiga1 y Hugo Campos de Quiroz2
 

The use of double haploid techniques in plant breeding has the potential of shortening genetic improvement cycles in comparison to conventional methods, by means of the fast production (one cycle) of homozygous lines derived from segregant populations, which facilitates rapid homogenization and genetic stabilization. The parental material used (F3) was previously selected under field conditions. A high level of efficiency was reached in obtaining embryos (31.1%), which were rescued and germinated in vitro cultivation to later, by means of duplication via colchicine, to obtain more than 1000 double haploid wheat plants.

Keywords: Triticum aestivum L., Zea mays L., double haploids, breeding, selection.
1 Instituto de Investigaciones Agropecuarias, Centro de Investigaciones Carillanca, Casilla 58-D, Temuco, Chile. E-mail: cjobet@inia.cl *Autor para correspondencia
2 Semillas Pioneer, Chile. E-mail: camposh@phibred.com