Integrated use of Rhizobium leguminosarum, plant growth promoting rhizobacteria and enriched compost for improving growth, nodulation and yield of lentil (Lens culinaris Medik.).

Muhammad Asif Iqbal1, MuhammAd Khalid1, Sher Muhammd Shahzad2*, Maqshoof Ahmad1, Nawaf Soleman3, and Naeem Akhtar4

Maintenance of high bacterial population in the rhizosphere improves the efficiency of the organisms. This high bacterial population can be maintained by the application of enriched compost which supports their growth and activities. Thus integrated use of Rhizobium, plant growth promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate deaminase (ACC-deaminase) and P-enriched compost (PEC) could be highly effective for promoting growth, nodulation, and yield of lentil (Lens culinaris Medik.). A field study was conducted to evaluate the potential of Rhizobium, PGPR containing ACC-deaminase and PEC for promoting growth of lentil. For this study, the soil type was sandy clay loam soil having pH 7.6; EC (electrical conductivity) 2.8 dS m-1; organic matter (OM) 0.59%; total N 0.032%; available P 7.9 mg kg-1, and extractable K 129 mg kg-1. Treatments were replicated thrice, using randomized complete block (RCB) design. Results showed that the integrated use of R. leguminosarum with Pseudomonas spp. containing ACC-deaminase along with PEC was highly effective and caused up to 73.5, 73.9, 74.4, 67.5, 73.3, 65.8, 40.5, and 52.5% increase in fresh biomass, grain yield, straw yield, pods plant-1, nodule plant-1, nodule dry weight plant-1, 1000-grain weight, and N content in grain of lentil, respectively, as compared to respective control. It is concluded that integrated use of R. leguminosarum with Pseudomonas spp. having trait ACC-deaminase plus PEC would be an effective approach for better nodulation which consequently improved yield of lentil under natural conditions.

Keywords: Co-inoculation, biological N fixation, Pseudomonas spp., rhizobium, ACC-deaminase, P-enriched compost, nodulation, yield, Lens culinaris.
1University of Agriculture Faisalabad, Institute of Soil & Environmental Sciences, Faisalabad, Pakistan.
2University of Sargodha, University College of Agriculture, Department of Soil & Environmental Sciences, Sargodha, Pakistan. *Corresponding author (smshahzad_uaf@yahoo.com).
3University of Wales, Environment Centre Wales, School of Environmental & Natural Resources, Bangor, UK.
4University of Sargodha, University College of Agriculture, Department of Plant Breeding and Genetics, Pakistan.