Cold tolerance evaluation of chilean rice genotypes at germination stage.
|Gabriel Donoso Ñanculao1*, Mario Paredes Cárcamo1, Oscar Arbiza de los Santos2, Viviana Becerra Velásquez1|
Low temperature is the most important abiotic stress affecting rice (Oryza sativa L.) yield in Chile. Rice in Chile is usually planted when the minimum air temperatures are below 12 °C. This temperature is lower than the optimum needed for normal rice germination. Therefore, the aim of this study was to evaluate cold tolerance in 29 experimental lines from the Rice Breeding Program of the Instituto de Investigaciones Agropecuarias (INIA), Chile at the germination stage. Coleoptile length reduction (CRED), coleoptile length after cold treatment (CLEN), coleoptile length recovery (CREC), and coleoptile regrowth (CREG) were evaluated at 13 °C for 4 d using ‘Diamante-INIA’ as the cold-tolerant control. To find genotypes with cold tolerance (low CRED value and high CLEN, CREC, and CREG values), genotypes were ranked, a biplot of principal components, and cluster analysis were performed. No differences were found among genotypes in the ranking based on CREC value so this trait was not considered. Analysis showed that only three experimental lines had cold tolerance similar to that of ‘Diamante-INIA’; all other experimental lines exhibited intermediate to low cold tolerance. These results showed low cold tolerance of some Chilean genotypes at the germination stage, thus confirming the need to evaluate the rest of the germplasm from the Rice Breeding Program.
|Keywords: Low temperatures, coleoptile length, experimental lines.|
|1Instituto de Investigaciones Agropecuarias, Av. Vicente Méndez 515, Chillán, Chile. *Corresponding author (email@example.com). |
2Universidad Adventista de Chile, Facultad de Ingeniería y Negocios, Camino las Mariposas km 12, Chillán, Chile.