Genetic variation within three populations of Phycella australis (Phil.) Ravenna from Biobío Region, Chile, evaluated using ISSR markers.

Cristian Flores1, Catherine Delaveau1, Carolina Alvarez1, Dafne Vergara1, Carlos Baeza2, Eduardo Ruiz2, Matilde Uribe3*

Phycella australis (Phil.) Ravenna is a Chilean plant with high ornamental potential; however, the intensive extraction as a cut flower might be detrimental for the conservational state by ignoring the state of genetic variation. The objective of this investigation was to assess genetic variability within and between three populations of P. australis in the Bio Bio Region using inter-simple sequence repeat (ISSR) markers. The evaluated areas correspond to three locations in the province of Concepción, Bio Bio Region: Desembocadura (36º48’ S, 73º10’ W), Santa Juana (36º58’ S, 72º58’ W), and Lipinhue (37º00’ S, 72º58’ W). Six ISSR primers were used obtaining 51 fragments, from which 72.5% were polymorphic. From the three evaluated sites Santa Juana showed a higher percentage of polymorphic loci (76.47%). From this variability, 83% belong to within population variability and only 17% belong to variability between populations. The dendrogram generated using the unweighted pair group method with arithmetic mean (UPGMA) method, grouped Lipinhue and Santa Juana sites together, which agrees with the geographic locations. This investigation proved that P. australis has high genetic variability despite the exploitation for economic purposes.

Keywords: Amaryllidaceae, genetic variability, molecular marker, ornamental plant.
1Universidad de Concepción, Centro de Biotecnología, Barrio Universitario s/n, Concepción, Chile.
2Universidad de Concepción, Facultad de Ciencias Naturales y Oceanográficas, Barrio Universitario s/n, Casilla 160-C; Concepción, Chile.
3Universidad de Concepción, Facultad de Ciencias Forestales, Barrio Universitario s/n, Casilla 160-C; Concepción, Chile. *Corresponding author (muribe@udec.cl).