ABSTRACT.
Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping

Jayamohan Subramanian1, and Kumudini Satyan1*
 
Fluorescent pseudomonads, acclaimed plant associated bacterial group, are well-known plant growth promoting-biocontrol agents in rhizosphere arena. In this study, 144 fluorescent pseudomonad isolates from rhizosphere soil samples were screened with King’s medium B supplemented with 8-hydroxyquinoline (8-HQ) chelator and comprehensively profiled for plant growth promotion viz., production of indole acetic acid (IAA), siderophore, ammonia, hydrogen cyanide, motility, phosphate solubilization, root growth promotion, and biofilm forming ability, along with two known control strains of pseudomonads. Iron and IAA regulated secondary metabolite siderophore production were investigated quantitatively. All isolates were positive for ammonia production and motility; 46% isolates were positive for hydrogen cyanide, 44% shown positivity for phosphate solubilization, and 40% isolates for siderophore production. Siderotyping showed production of hydroxamate type of siderophores which are known to be more efficient biocontrol agents. All isolates stimulated root growth to varying extent and had potentiality to form biofilms, a critical constituent for survival on different environments. Forty-two isolates of pseudomonads showed antagonistic behavior against the deleterious fungal pathogen Fusarium oxysporum (MTCC1755). Based on the above observations and statistical analysis, 11 isolates were shortlisted for further scrutiny. The study of biogeographic correlation and secondary metabolite profiling in association with plant growth promotion focalizes significant assessment on the behavior and antagonistic action, which probably brings out a competent biocontrol agent in a sustainable eco-friendly dimension.
Keywords: IAA, PGPR, plant growth promotion, rhizosphere, siderophore
1Jain University, Department of Biotechnology, Jayanagar, Bangalore 560011, India. *Corresponding author (kumudini_satyan@yahoo.co.in).