Multiple marker-traits associations for maize agronomic traits

Sanja Mikic1*, Ankica Kondic Spika1, Ljiljana Brbaklic2, Dusan Stanisavljevic1, Dragana Trkulja1, Marina Tomicic1, Aleksandra Nastasic1, Borislav Kobiljski2, Slaven Prodanovic3, and Gordana Surlan Momirovic3
Association analysis is a relatively novel approach in quantitative traits studies that allows high resolution mapping and time efficient and direct application on breeding material. Since the markers, which are close to the quantitative trait loci stable across environments and genetic backgrounds, may be valuable for marker assisted selection, we chose microsatellite markers previously linked to traits of interest in various mapping studies. A set of 36 microsatellite markers positioned near important maize (Zea mays L.) agronomic loci was used to evaluate genetic diversity and determine population structure. To verify the associations between the markers and traits, a panel of diverse maize inbred lines was genotyped with microsatellites and phenotyped for flowering time, yield and yield components. A relatively high level of polymorphism detected in number of alleles per locus (8.2), average polymorphic information content value (0.64), and average gene diversity (0.684) lines showed the analyzed panel of maize inbred contained significant genetic diversity and was suitable for association mapping. The population structure estimated by model-based clustering method grouped maize inbred lines into three clusters. The association analysis using the general linear and mixed linear models determined significant correlations between several agronomic traits and three microsatellites on chromosomes 3, 5, and 8, namely umc1025, bnlg1237, and bnlg162 consistent across the environments, explaining from 4.7% to 18.2% of total phenotypic variations. The results suggest that the chromosome regions containing quantitative trait loci (QTLs) associated with multiple yield-related traits consistently across environments are potentially important targets for selection.
Keywords: Association analysis, inbred lines, linkage, pleiotropy, SSR markers, Zea mays.
1Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia.
*Corresponding author (sanja.mikic@ifvcns.ns.ac.rs).
2Biogranum, Toplice Milana 20, 21000 Novi Sad, Serbia.
3University in Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia.