Optimization of media formulation for axillary shoot multiplication of the red-peeled sweet potato, (Ipomoea batatas [L.] Lam.) 'Abees'

Yaser Hassan Dewir1, 2*, Abdulhakim A. Aldubai1, 3, Mafatlal M. Kher4, Abdullah A. Alsadon1, Salah El-Hendawy1, 5, and Nasser A. Al-Suhaibani1
Sweet potato (Ipomoea batatas [L.] Lam.) is an important food crop that is cultivated and consumed worldwide, and its nutritional value makes it especially important in developing countries. However, sweet potato plants produced using conventional vegetative propagation can be prone to several diseases, thereby hindering crop yield. Therefore, micropropagation techniques are useful for producing higher quality plant material. The aim of the present study was to determine the optimal medium formulation for the axillary shoot multiplication of single-node ‘Abees’ explants. The influences of cytokinins (6-benzyladenine [BA], kinetin, thidiazuron, and zeatin), gibberellic acid, phenylacetic acid, indole-3-butyric acid, silver nitrate, and oxalic acid, culture duration, and medium salt strength were investigated. Optimal proliferation was observed when shoots were cultured on half-strength MS medium that was supplemented with 2 mg L-1 BA and 100 mg L-1 oxalic acid for 2 wk, followed by culture on half-strength MS medium without plant growth regulators for 4 wk. In this medium, the greatest number of shoots (3.1) and total number of nodes (16.1) per explant were observed. All (100%) of the regenerated shoots were rooted and acclimatized, with a survival rate of 96% when transferred to ex vitro conditions and no morphological abnormalities.
Keywords: Convolvulaceae, culture duration, micropropagation, oxalic acid, phenylacetic acid, silver nitrate.
1King Saud University, College of Food & Agriculture Sciences, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
*Corresponding author (ydewir@ksu.edu.sa).
2Kafrelsheikh University, Faculty of Agriculture, Kafr El-Sheikh 33516, Egypt.
3Agriculture Research and Extension Authority, Sana’a 87148, Yemen.
4Sardar Patel University, P.G. Department of Biosciences, Sardar Patel Maidan, Vadtal Road, Vallabh Vidyanagar, Gujarat 388120, India.5Suez Canal University, Faculty of Agriculture, Ismailia 41522, Egypt.