Establishment of callus culture of Melientha suavis Pierre

Duangporn Premjet1, Abraham K. Obeng2, and Siripong Premjet1, 3*
Melientha sauvis Pierre is an edible wild tree in Thailand. Local communities in Thailand harvest new shoot of the plant by burning the forest to stimulate it, a process that destroys the forest. This study was aimed at developing a methodology for callus culture production of M. suavis. Callus was induced from leaf, young stem, and shoot tip of M. suavis on Murashige and Skoog (MS) basal medium supplemented with various formulations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (6-BA). Callus produced from young stem explants appeared faster (14 d). Maximum callus growth (40%) and fresh weight (5.56 g) were observed in young stem explant cultured on MS medium containing 2 mg L-1 2,4-D. The findings of this work contribute to large scale micropropagation or bioactive compound production in M. suavis.
Keywords: 6-Benzylaminopurine, callus cultures, 2,4-dichlorophenoxyacetic acid, pak wan pa.
1Naresuan University, Faculty of Agriculture, Natural Resources and Environment, Center of Excellence in Research for Agricultural Biotechnology, Muang, Phitsanulok, 65000, Thailand.2University for Development Studies, Faculty of Agriculture, Department of Biotechnology, P.O. Box TL 1882, Tamale, Ghana.3Naresuan University, Faculty of Science, Department of Biology, Muang, Phitsanulok 65000, Thailand.*Corresponding author (siripongp@nu.ac.th).