Occurrence and molecular characterization of Potyvirus present in the garlic crop in the Mediterranean ecosystem of the central valley of Chile

Mónica Madariaga1*, Isabel Ramírez1, Rodrigo Lizana2, Nelly Nova1, Adolfo Donoso1, and Valentina Torrejón2
The garlic (Allium sativum L.) cultivated in the O’Higgins Region, the main garlic-producing area in Chile, is the commercial type known as pink garlic, which presents a low yield and a high prevalence of symptoms associated with viral infections. A survey was carried out in the three localities of the region that cultivate this vegetable to identify, by reverse transcription polymerase chain reaction (RT-PCR), the presence of Onion yellow dwarf virus (OYDV) and Leek yellow stripe virus (LYSV). The results indicated a prevalence of 99.5% and 54.0% respectively. In addition, a molecular and phylogenetic analysis of the amplified genome fragments for both viruses was performed. Using the nucleotide BLAST (BLASTn) tool, nucleotide sequences were compared with sequences available in the National Center for Biotechnology Information (NCBI) database. The Chilean sequences were aligned with complete sequences available at NCBI and a phylogenetic tree was constructed using the Neighbor-Joining method. The results showed genetic variability among Chilean isolates.
Keywords: Garlic, LYSV, OYDV, Potyvirus.
1Instituto de Investigaciones Agropecuarias, INIA La Platina, Santa Rosa 11610, La Pintana, Santiago, Chile.
2Universidad Andrés Bello, Facultad Ciencias de la Vida, República 440, Santiago, Chile.
*Corresponding author (mmadariaga@inia.cl).