Evaluation of Chilean strains of Metarhizium anisopliae var. anisopliae against Otiorhynchus sulcatus Fab. (Coleoptera: Curculionidae).

Macarena Gerding G., Andrés France I. 1 y Ernesto Cisternas A.

The pathogenicity of three isolates of Metarhizium anisopliae var. anisopliae against the black vine weevil (Otiorhynchus sulcatus) was evaluated in a laboratory test. The isolates were applied in increasing concentrations (0 to 108 conidia mL-1) to the last instar larvae. Larval mortality was evaluated for 20 days, and lethal concentration LC50 LC90, and mortality rates were calculated. The three isolates varied (P< 0.01) in the rate of mortality at different concentrations. The LC50 and LC90 for the most virulent isolate were about 106 and 108 conidia mL-1, respectively. Future field studies will determine the potential of this fungus as a biological control agent for Otiorhynchus sulcatus in Chile.

Keywords: biological control, black vine weevil, entomogenous fungi, insect pathology.
1 Instituto de Investigaciones Agropecuarias, Centro Regional de Investigación Quilamapu, Casilla 426, Chillán, Chile. E-mail: afrance@inia.cl.