Identification of Chilean rice (Oryza sativa L.) cultivars: evaluation of protein profiles and Random Amplified Polymorphic DNA (RAPO)
|Patricio Hinrichsen R.1, Cecilia Amigo G.2, Roberto Alvarado A.2 y Carlos Muñoz S.1|
Proper cultivar identification is essential for registration and control during seed marketing and for assessing the purity of a seed lot. In the present work, various molecular marker techniques were used to discriminate rice cultivars currently used in Chilec Electrophoretic analysis of seed proteins showed identical profiles for 6 different cultivars, both in non-native (SOS-PAGE) or acidic (A-PAGE) conditions. On the contrary, when direct genomic analysis was performed using randomly amplified polymorphic ONA, cultivars were easily distinguishable. Ten out of 22 tested primers generated ONA polymorphic bands. Bands generated by 4 of these were used to design a protocol for cultivar identification in rice. Additionally, detected polymorphisms were used to make a dendrogram that could estimate the phylogenetic relationships among the cultivars included in this study.
|Keywords: RAPO, SOS-PAGE, A-PAGE, rice phylogenetics.|
|1 Centro Regional de Investigación La Platina (INIA), Casilla 439, Correo 3, Santiago, Chile. |
2 Centro Regional de Investigación Quilamapu (INIA), Casilla 426, Chillén, Chile.