In vitro propagation of Gypsophila paniculata L.
|Mónica Castro V.1, Christine Dardel H.1 y Gabriela Verdugo R.1|
A series of experiments were carried out in order to establish optimal nutrient levels for Gypsophila paniculata propagated in vitro from meristem tips. Basal medium was 1/2 of Murashige y Skoog (1962) (MS) plus different combinations of IAA and kinetin. The same media were tested for phase I (establishment) and phase II (proliferation). No differences were observed for phase I and for number of shoots in phase II. However shoots were longest at highest level of auxin (V5 medium). Maximum vitrification rate (69%) was obtained when used 10 mg/L of IAA and 2 mg/L of kinetin. Rooting was done in vivo. Two rooting media were tested, sand and sand peatmoss (3:1 v/v). No differences were observed in rooting percentage, but greater number of roots per plants were observed with the peat enriched medium. From one single explant, 7 plantlets were obtained during five months of culture.
|Keywords: tissue culture, micropropagation, Gypsophila paniculata.|
|1 Facultad de Agronomía, Universidad Católica de Valparaíso, Casilla 4-D, Quillota, Chile.|